Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 1 The map of the recombinant vector for expression of His-CDH1 fusion peptide. The CDH1 ectodomain was link to the His-tag in the pET-44a(+) vector. Expression of His-CDH1 fusion peptide was driven by T7 lac promoter.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Recombinant, Plasmid Preparation, Expressing

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 2 Analysis of the optimal expressed condition of His-CDH1 fusion peptide by SDS-PAGE. M, protein molecular weight marker; 1, control; 2-9, the sample induced by IPTG at 1, 1.5, 2, 2.5, 3, 4, 5 and 6 h, respectively.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: SDS Page, Molecular Weight, Marker, Control

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 3 Western blot analysis of the condition for purifying His- CDH1 peptide using Ni-NTA chromatography. 1, prestained protein marker; 2, the recovery fluid with lysate supernatant; 3, the recovery fluid with 20 mmol imidazol; 4-5, the recovery fluid with 100 mmol imidazol; 6-7, the recovery fluid with 250 mmol imidazol; 8-9, the recovery fluid with 500 mmol imidazol; 10, the recovery fluid with 1 000 mmol imidazol.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot, Chromatography, Marker

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 4 Ability of the sheep CDH1 polyclonal antibodies was detected by Western blotting. Total protein was from sheep testis (T), kinedy (K), pulmo (P) and liver (L). There were three bands in 135, 120 and 80 kDa.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 5 Distribution patterns of CDH1 and PLZF in cross sections of 5-mon-old sheep testis. A, immunohistochemistry examination of PLZF expressing cells within cross sections of seminiferous tubules from 5-mon-old sheep testis. B, immunohistochemistry examination of CDH1 in the same section. C, the nuclei of the cell sections were stained by Hoechst 33342. D, merged image of A, B and C. Some PLZF- and CDH1-positive cells comprising single (diamond arrows) and paired spermatogonia (arrows) were localized at the basement membrane of the seminiferous tubules. Scale bars are 40 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Expressing, Staining, Membrane

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 6 Whole-mount immunohistochemistry of 5-mon-old sheep seminiferous tubules. The CDH1-positive cells were small in number, there are single cells attached the basement membrane of the seminiferous tubules (arrows), and there are paired cells attached each other (diamond arrow). Scale bar is 200 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Membrane

Journal: Journal of Integrative Agriculture

Article Title: Critical role of cytochrome c1 and its cleavage in porcine reproductive and respiratory syndrome virus nonstructural protein 4-induced cell apoptosis via interaction with nsp4

doi: 10.1016/s2095-3119(17)61670-8

Figure Lengend Snippet: Fig. 1 Porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 4 (nsp4) interacts with cytochrome c1 (cyto.c1). A, yeast two hybrid assay. The plasmids pGBKT7-nsp4 and pGADT7-cyto.c1 were co-transformed into the yeast strain Y2HGold and selected on QDO/X/ABA plates. The top and second panels are the respective positive and negative controls. B, HEK 293T cells were transfected to express Flag-cyto.c1, or HA-nsp4 or together. At 24 h post-transfection, the cells were either subjected to direct Western blot analysis or lysed and immunoprecipitated with anti-HA antibodies. The proteins bound to sepharose beads were separated by SDS-PAGE, transferred to PVDF membranes, and probed with the antibodies to Flag and HA. C, the same as B except that nsp4 truncation mutants were used. D, MARC-145 cells were transduced with letiviruses that were expressing GFP or nsp4-GFP in the presence of 8 µg mL–1 polybrene, respectively. The cells were harvested 48 h post-transduction and cell lysates were immunoprecipitated using anti-GFP beads. The proteins bound to sepharose beads were subjected to Western blot analysis using an anti-cytochrome c1 polyclonal antibody or anti-GFP mAb. E, PRRSV nsp4 interacts with cyto.c1 in mammalian cells. Vero cells grown on coverslips in six-well plates were transfected when 60–70% to express the indicated proteins, either alone or pairwise. At 18–24 h post-transfection, the cells were fixed, permeablized, and stained with antibodies to HA and Flag, and examined by confocal microscopy. For double transfections, the merged images are shown at the right.

Article Snippet: Mouse anti-green fluorescent protein (GFP) mAb (66002-1-Ig) and rabbit anti-cytochrome c1 polyclonal antibodies (10242-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques: Virus, Y2H Assay, Transformation Assay, Transfection, Western Blot, Immunoprecipitation, SDS Page, Transduction, Expressing, Staining, Confocal Microscopy

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 2. NF-kBp65 (A), cyclo-oxygenase-2 (COX-2) (B) and inducible NO synthase (iNOS) (C) expressions in the liver. m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: British Journal of Nutrition

Article Title: Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism

doi: 10.1017/s0007114511001322

Figure Lengend Snippet: Fig. 5. Hepatic mRNA expressions of acetyl-CoA carboxylase (ACC) (A), fatty acid synthase (FAS) (B) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) (C). m/m, Misty; db/db, diabetic; Veh, db/db vehicle-treated mice; Oligo-10, db/db mice treated with oligonol at 10 mg/kg body weight; Oligo-20, db/db mice treated with oligonol at 20 mg/kg body weight. Values are means (n 6 or n 10), with standard errors represented by vertical bars. a,b Mean values with unlike letters were significantly different (P,0·05; Duncan’s test).

Article Snippet: Rabbit polyclonal antibodies against PPARa, SREBP-1, SREBP-2, NF-kBp65 and RAGE, and mouse monoclonal antibody against cyclo-oxygenase-2 (COX-2) and inducible NO synthase (iNOS) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: British Journal of Nutrition

Article Title: Chronic ingestion of deoxynivalenol and fumonisin, alone or in interaction, induces morphological and immunological changes in the intestine of piglets

doi: 10.1017/s0007114511004946

Figure Lengend Snippet: Fig. 5. Effect of individual and combined deoxynivalenol (DON) and fumonisins (FB) exposure on the intestinal expression of E-cadherin. Pigs received a control diet ( ), or a diet contaminated with DON ( ), FB ( ), or both DON and FB ( ). (A) Jejunum of a control piglet showing a strong and (B) homogeneous immunor- eactivity to E-cadherin. Immunoperoxidase, 20£ . (C) Percentage of animals showing strong immunoreactivity to E-cadherin. a,b Mean values with unlike letters were significantly different (P,0·05).

Article Snippet: The antibodies used in the present study were E-cadherin (24E10) rabbit mAb (diluted 1:500; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-occludin (672381A, diluted 1:500; Invitrogen, Cergy-Pontoise, France) and b-actin mouse mAb (8H10D10; Cell Signaling).

Techniques: Expressing, Control